Re: setThreshold Macro command for fluorescent microscopy

Posted by Morange on
URL: http://imagej.273.s1.nabble.com/setThreshold-Macro-command-for-fluorescent-microscopy-tp5016342p5016367.html

Hello Gabriel,

Thank you for your reply. I tried your suggestion in the macro and this time, the image is only inverted and converted to 8-bit before it was measured, it appears that setThreshold was not called

run("Invert");
run("8-bit");
setThreshold(0, 162, "Red")
run("Measure");