Re: Apply bUnwarpJ in macro

Posted by nellypledge on May 10, 2016; 11:44am
URL: http://imagej.273.s1.nabble.com/Apply-bUnwarpJ-in-macro-tp5016374p5016383.html

Hi Guys, thanks for the feedback.

The one things that keeps throwing me is the fact you need the target and source repeatedly.  Surely once I've created a transform file I can apply it to one image (I'm assuming that I'd apply a forward transform to a source image, right?).  Why don't we just have the source, transform and output?

Maybe I'm missing something...  For your reference I want to use this plugin to map my green channel camera image back to the red channel.  I acquired images with a full field of mutlicolor beads to create the transform.  Using the target, source, transform and output, how would that look?  Would the target and source be the bead image of red and green respectively, and then the transform acts on the output file?

I would be applying this to potentially large zstacks and would probable need the memory adapted version.  I'm happy to work with github to keep things current.  Philippe, if you plan to update the repository, I'll wait for you, but if you foresee potential time delays, I'm happy to figure that out and update things.

Thanks
Neil