Posted by
Thomas Boudier-4 on
May 19, 2016; 7:23am
URL: http://imagej.273.s1.nabble.com/Problem-with-3D-Objects-Counter-tp5016458p5016465.html
Hi Maria,
Did you check that the objects are not touching the edges. If you
checked "exclude objects on edges" it will also remove the objects
touching the edges in Z, meaning objects is first or last slice will be
removed.
hope this helps
best,
Thomas
On 18/05/2016 22:42, María Victoria Pepe wrote:
> Hi, I'm having a problem with the 3D Object counter tool. I´m working with
> Z-stacks of around 0,5 Gigabyte, and I've noticed that some objects present
> in the original image, do not show up in the object map, even though their
> fluorescence intensity is way above the threshold and the size is similar
> to other objects that appear in the object map
>
> What can Ido?
>
> Thanks
>
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http://imagej.nih.gov/ij/list.html>
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Thomas Boudier, Associate Professor, UPMC,
Université Pierre et Marie Curie, Paris, France.
BioInformatics Institute (BII)/IPAL, Singapore.
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