Merge Multiple Images & Cut Through all of Them

Posted by Marina on Jun 09, 2016; 2:58pm
URL: http://imagej.273.s1.nabble.com/Merge-Multiple-Images-Cut-Through-all-of-Them-tp5016617.html

Hello All,

I am doing fluorescence microscopy and have four fluorescence channels, each saved as a tiff file.
I wish to merge these files, cut out objects in the same place for each one, then split them again.

When I had only three channels, this was easy: Image > Color > Merge Channels, use the lasso and backspace to edit what I needed and then Image > Color > Split Channels.
However I cannot do this with four channels as Split Channels only splits into red, green and blue despite me merging with red, green, blue and grey.

The solution I have come up with it to form a stack with my images instead of merging and then use the lasso and Edit > Clear to delete the selection in every channel, however this comes up with an "are you sure?" dialogue box and is tedious as I have many cuts and many images to process.

Is there a way for me to split my merged tiffs back into their four channels?
Or does anyone know of another solution to ensure that my cut outs are in the same place for all my channels?

Many thanks,

Marina