Posted by
Swayne, Theresa C. on
Jun 09, 2016; 7:05pm
URL: http://imagej.273.s1.nabble.com/Merge-Multiple-Images-Cut-Through-all-of-Them-tp5016617p5016618.html
Hi Marina,
When you merge channels, check the box “Create Composite”.
Then you will have an image with a slider at the bottom where you can select each channel in turn to do the editing.
The composite image preserves the channel information in your original images.
As a side benefit, the ROI you draw with the lasso will be in the same place automatically.
As an alternative, to generate an ROI in the same place in multiple images, you can use the ROI Manager.
(user guide:
https://imagej.nih.gov/ij/docs/guide/146-30.html#sub:ROI-Manager…)
After you draw the ROI, press T to add it to the manager.
To apply the ROI to another image, click on the other image window, then click on the ROI name in the ROI Manager.
Hope this helps,
Theresa
On Jun 9, 2016, at 10:58 AM, Marina <
[hidden email]<mailto:
[hidden email]>> wrote:
When I had only three channels, this was easy: Image > Color > Merge
Channels, use the lasso and backspace to edit what I needed and then Image >
Color > Split Channels.
However I cannot do this with four channels as Split Channels only splits
into red, green and blue despite me merging with red, green, blue and grey.
…
Or does anyone know of another solution to ensure that my cut outs are in
the same place for all my channels?
------------------------------------
Theresa Swayne, Ph.D.
Manager
Confocal and Specialized Microscopy Shared Resource<
http://hiccc.columbia.edu/research/sharedresources/confocal>
Herbert Irving Comprehensive Cancer Center
Columbia University Medical Center
1130 St. Nicholas Ave., Room 222A
New York, NY 10032
Phone: 212-851-4613
[hidden email]<mailto:
[hidden email]>
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