http://imagej.273.s1.nabble.com/Merge-Multiple-Images-Cut-Through-all-of-Them-tp5016617p5016619.html
Are you merging your channels into Composite Hyperstacks? Image>Color>Merge Channels, make certain the ‘Create Composite’ box is checked. All selection operations are possible with composite stacks.
Alternatively on single channel images, create your selection on 1 channel, open the ROIManager and click on ‘Add (t)’ . Now select each channel in turn, click on the ROI to apply it and perform your operation. Finally, merge the channels.
> On Jun 9, 2016, at 7:58 AM, Marina <
[hidden email]> wrote:
>
> Hello All,
>
> I am doing fluorescence microscopy and have four fluorescence channels, each
> saved as a tiff file.
> I wish to merge these files, cut out objects in the same place for each one,
> then split them again.
>
> When I had only three channels, this was easy: Image > Color > Merge
> Channels, use the lasso and backspace to edit what I needed and then Image >
> Color > Split Channels.
> However I cannot do this with four channels as Split Channels only splits
> into red, green and blue despite me merging with red, green, blue and grey.
>
> The solution I have come up with it to form a stack with my images instead
> of merging and then use the lasso and Edit > Clear to delete the selection
> in every channel, however this comes up with an "are you sure?" dialogue box
> and is tedious as I have many cuts and many images to process.
>
> Is there a way for me to split my merged tiffs back into their four
> channels?
> Or does anyone know of another solution to ensure that my cut outs are in
> the same place for all my channels?
>
> Many thanks,
>
> Marina
>
>
>
> --
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