Re: Merge Multiple Images & Cut Through all of Them

Posted by Jan Eglinger on Jun 09, 2016; 7:17pm
URL: http://imagej.273.s1.nabble.com/Merge-Multiple-Images-Cut-Through-all-of-Them-tp5016617p5016620.html

Hi all,

another way to transfer an ROI from one image to another is the
   'Edit > Selection > Restore Selection'
command.

* Activate the image containing the desired selection (by clicking the
title bar or using your OS's mechanism to cycle through windows, e.g.
Alt-Tab)

* Activate the image where you want to transfer the ROI

* Press Shift-E

Done.
Hope that helps,
Jan



On 09.06.16 21:20, Swayne, Theresa C. wrote:

>
> Hi Marina,
>
> When you merge channels, check the box “Create Composite”.
>
> Then you will have an image with a slider at the bottom where you can select each channel in turn to do the editing.
>
> The composite image preserves the channel information in your original images.
>
> As a side benefit, the ROI you draw with the lasso will be in the same place automatically.
>
> As an alternative, to generate an ROI in the same place in multiple images, you can use the ROI Manager.
> (user guide: https://imagej.nih.gov/ij/docs/guide/146-30.html#sub:ROI-Manager…)
>
> After you draw the ROI, press T to add it to the manager.
>
> To apply the ROI to another image, click on the other image window, then click on the ROI name in the ROI Manager.
>
> Hope this helps,
> Theresa
>
>
> On Jun 9, 2016, at 10:58 AM, Marina <[hidden email]<mailto:[hidden email]>> wrote:
>
> When I had only three channels, this was easy: Image > Color > Merge
> Channels, use the lasso and backspace to edit what I needed and then Image >
> Color > Split Channels.
> However I cannot do this with four channels as Split Channels only splits
> into red, green and blue despite me merging with red, green, blue and grey.
>
>
>
> …
>
> Or does anyone know of another solution to ensure that my cut outs are in
> the same place for all my channels?
>
> ------------------------------------
> Theresa Swayne, Ph.D.
> Manager
> Confocal and Specialized Microscopy Shared Resource<http://hiccc.columbia.edu/research/sharedresources/confocal>
>
> Herbert Irving Comprehensive Cancer Center
> Columbia University Medical Center
> 1130 St. Nicholas Ave., Room 222A
> New York, NY 10032
> Phone: [phone]
> [hidden email]<mailto:[hidden email]>
>
>
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>

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