Posted by
Swayne, Theresa C. on
Jun 14, 2016; 5:31pm
URL: http://imagej.273.s1.nabble.com/Merge-Multiple-Images-Cut-Through-all-of-Them-tp5016617p5016641.html
Hi Marina,
You can merge 4 channels if they are each single-color images in “grayscale” format (says "8-bit" or "16-bit" at the top of the image window, NOT “RGB").
It could be that your images are already RGB format before merging.
If you convert them to 8-bit or 16-bit before merging, do you still have the problem?
If that doesn’t solve it, could you post some sample images on imgur or on the forum (forum.imagej.net<
http://forum.imagej.net>)?
Clearing areas is channel-specific in composite images, on purpose. You will have to scroll through the channels and press delete on each one. The advantage is that your lasso selection stays in the same place automatically.
Hope this helps.
Best,
Theresa
On Jun 14, 2016, at 11:47 AM, Marina <
[hidden email]<mailto:
[hidden email]>> wrote:
The reason I have not used composities is that when I create a composite it
only has three channels, despite me including four in the "Merge Channels"
option. I wish to analyse each channel separately after cutting out the
areas I wish to exclude.
Also: when I use the lasso on one channel and hit "delete" it only deletes
the area on that channel, as opposed to on all three.
Best wishes,
Marina
Swayne, Theresa C. wrote
When you merge channels, check the box “Create Composite”.
Then you will have an image with a slider at the bottom where you can
select each channel in turn to do the editing.
The composite image preserves the channel information in your original
images.
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Theresa Swayne, Ph.D.
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Confocal and Specialized Microscopy Shared Resource<
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Herbert Irving Comprehensive Cancer Center
Columbia University Medical Center
1130 St. Nicholas Ave., Room 222A
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