Re: Overlay on image stack

Posted by AAM71 on Jun 20, 2016; 10:36am
URL: http://imagej.273.s1.nabble.com/Overlay-on-image-stack-tp5016634p5016695.html

Dear Wayne,

Thank you very much for all your help with bugs and macros!

Sincerely,
Alex


On 17/06/2016 04:16, Rasband, Wayne (NIH/NIMH) [E] wrote:

>> On Jun 13, 2016, at 8:29 AM, Aleksandr Mironov <[hidden email]> wrote:
>>
>> Dear All,
>>
>> Is it possible to use different or alternating non-destructive overlays on different slices of a stack? Or for each slice the overlay should be flattened? For example, I want to put an overlay of parallel lines so that for each slice of a stack line orientation will alternate between two directions.
> You can do this. The following macro provides an example.
>
> -wayne
>
>    // Create a 4 slice stack
>    newImage("Overlay", "8-bit black", 500, 500, 4);
>    // Use Image>Overlay>Overlay Options to
>    // specify the line color and width
>    run("Overlay Options...", "stroke=red width=5 set”);
>    // Add a line to the overlay in each of the 4 slices
>    makeLine(117, 98, 385, 379);
>    run("Add Selection...");
>    run("Next Slice [>]");
>    makeLine(368, 100, 117, 405);
>    run("Add Selection...");
>    run("Next Slice [>]");
>    makeLine(93, 82, 365, 367);
>    run("Add Selection...");
>    run("Next Slice [>]");
>    makeLine(365, 77, 133, 372);
>    run("Add Selection...");
>    run("Select None”);
>    // Use Image>Overlay>List Elements to summarize
>    // the overlay. Note how the stack position of each
>    // line is shown in the “Pos” (position) column.
>    run("List Elements”);
>    // Use Image>Overlay>Flatten to flatten the stack
>    run("Duplicate...", "title=Flattened duplicate");
>    run("Flatten", "stack");
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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