Posted by
Aryeh Weiss on
Jun 29, 2016; 10:44am
URL: http://imagej.273.s1.nabble.com/stitching-RGB-images-tp5016749p5016755.html
Thanks to Stephen and Peter for their replies.
On 29/06/2016 6:34 AM,
[hidden email] wrote:
> Hi, the plugin uses Composite Images that instead of the wasteful and
> inflexible RGB images (using 32 bits per pixel where it is only 24 bit
> that are used for 3 channels and even only 16bit for 2 channels).
>
It happens that the NIS Elements software that generates the images
conveniently names them tile_x{xxx}_y{yyy}.tif, so I was ableto feed
them directly into the stitching program. However, it creates RGB, and
does not know from composite, so it would be convenient for the
stitching program, which anyway is creating all three channels, to
produce a composite output.
However, if it will not do that, I can write a macro that will convert
the input images to composite. It is just a step I hoped I could skip.
> You can always convert it later back to RGB if you like: Image > Type
> > RGB color.
>
I feed the output into the color deconvolution plugin (thank you
Gabriel), so I will see if that ill take composite images or wants RGB.
> Hope this helps,
Indeed it did. Thanks again.
--aryeh
> Stephan
> ---
>
> Dr. Stephan Preibisch
> Group Leader
>
> Berlin Institute of Medical Systems Biology of the MDC
> Building 89, 1.08b
>
> email:
[hidden email]
> <mailto:
[hidden email]>
> web:
http://www.preibisch.net/ <
http://fly.mpi-cbg.de/preibisch>
>
>> On 28 Jun 2016, at 17:20, Aryeh Weiss <
[hidden email]
>> <mailto:
[hidden email]>> wrote:
>>
>> I have 70 color TIFF images which I am stitching, using the excellent
>> grid/collection stitching plugin.
>>
>> It does a great job, and I see that it processes all of the colors.
>> However, it then produces an 8-bit color image, instead of the
>> expected RGB image. I can actually see all three images being
>> created, but then they are combined into a single 8-bit image.
>>
>> Is there a way to have it create an RGB fused image? Or, can I get at
>> the three images that it creates before merging them into an 8-but
>> color image?
>>
>> --aryeh
>>
>> --
>> Aryeh Weiss
>> Faculty of Engineering
>> Bar Ilan University
>> Ramat Gan 52900 Israel
>>
>> Ph: 972-3-5317638
>> FAX: 972-3-7384051
>>
>> --
>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel
Ph: 972-3-5317638
FAX: 972-3-7384051
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html