Posted by
Stephan.Preibisch@mdc-berlin.de on
Jun 29, 2016; 11:31am
URL: http://imagej.273.s1.nabble.com/stitching-RGB-images-tp5016749p5016758.html
Hi,
no, not if it is a Composite image, it then computes the average intensity of all channels and uses this for computing the alignment. Only if you provide each channel as separate image (e.g. one TIFF each), then, this will happen.
All the best,
Stephan
---
Dr. Stephan Preibisch
Group Leader
Berlin Institute of Medical Systems Biology of the MDC
Building 89, 1.08b
email:
[hidden email]<mailto:
[hidden email]>
web:
http://www.preibisch.net/<
http://fly.mpi-cbg.de/preibisch>
On 29 Jun 2016, at 07:27, Peter Haub <
[hidden email]<mailto:
[hidden email]>> wrote:
Hi Stephan,
when I remember correctly from my work with Composite Images the alignment is done on every channel. This leads to stitching artifacts when working with RGB images because the registration is different for the different channels.
Peter
On 29.06.2016 12:34,
[hidden email]<mailto:
[hidden email]> wrote:
Hi, the plugin uses Composite Images that instead of the wasteful and inflexible RGB images (using 32 bits per pixel where it is only 24 bit that are used for 3 channels and even only 16bit for 2 channels).
You can always convert it later back to RGB if you like: Image > Type > RGB color.
Hope this helps,
Stephan
---
Dr. Stephan Preibisch
Group Leader
Berlin Institute of Medical Systems Biology of the MDC
Building 89, 1.08b
email:
[hidden email]<mailto:
[hidden email]><mailto:
[hidden email]>
web:
http://www.preibisch.net/<
http://fly.mpi-cbg.de/preibisch><
http://www.preibisch.net/%3Chttp://fly.mpi-cbg.de/preibisch%3E>
On 28 Jun 2016, at 17:20, Aryeh Weiss <
[hidden email]<mailto:
[hidden email]><mailto:
[hidden email]>> wrote:
I have 70 color TIFF images which I am stitching, using the excellent grid/collection stitching plugin.
It does a great job, and I see that it processes all of the colors. However, it then produces an 8-bit color image, instead of the expected RGB image. I can actually see all three images being created, but then they are combined into a single 8-bit image.
Is there a way to have it create an RGB fused image? Or, can I get at the three images that it creates before merging them into an 8-but color image?
--aryeh
--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel
Ph: 972-3-5317638
FAX: 972-3-7384051
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