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3D Spot Segmentation

Posted by Feriel P on Jul 05, 2016; 5:52pm
URL: http://imagej.273.s1.nabble.com/3D-Spot-Segmentation-tp5016817.html

Hi guys,

Has anyone else had any issues with the 3D Image Suite's 3D Spot
Segmentation plugin? I have been trying to run it on many images through a
macro to generate an object count, then use the extension
Ext.Manager3D_Count(nb_obj); to get the count from the 3D ROI Manager and
assign it to a variable. This line worked fine in the macro yesterday, but
today it throws an error window that says "Unrecognized Ext function
Ext.<Manager3D_Count>(nb_obj) in line...". I also tried to run the 3D Spot
Segmentation plugin outside the macro but the 3D ROI Manager did not open
and I got the Exception window message below. I am still a novice at
ImageJ, so if anyone has any suggestions on what the issue is/what I can do
to get this plugin and its functions running again I would greatly
appreciate it!

Thanks,
-Feriel

*Error Message:*

(Fiji Is Just) ImageJ 2.0.0-rc-43/1.51e18; Java 1.6.0_24 [32-bit]; Windows
7 6.1; 239MB of 1211MB (19%)

java.lang.NoClassDefFoundError: org/scijava/java3d/View
 at java.lang.Class.getDeclaredMethods0(Native Method)
 at java.lang.Class.privateGetDeclaredMethods(Class.java:2427)
 at java.lang.Class.getDeclaredMethod(Class.java:1935)
 at java.awt.Component.isCoalesceEventsOverriden(Component.java:5974)
 at java.awt.Component.access$500(Component.java:170)
 at java.awt.Component$3.run(Component.java:5928)
 at java.awt.Component$3.run(Component.java:5926)
 at java.security.AccessController.doPrivileged(Native Method)
 at java.awt.Component.checkCoalescing(Component.java:5925)
 at java.awt.Component.<init>(Component.java:5894)
 at java.awt.Container.<init>(Container.java:251)
 at java.awt.Window.<init>(Window.java:431)
 at java.awt.Frame.<init>(Frame.java:403)
 at java.awt.Frame.<init>(Frame.java:368)
 at javax.swing.JFrame.<init>(JFrame.java:158)
 at mcib_plugins.tools.RoiManager3D_2.<init>(RoiManager3D_2.java:113)
 at mcib_plugins.SeedSpots_Plus.Segmentation(SeedSpots_Plus.java:266)
 at mcib_plugins.SeedSpots_Plus.run(SeedSpots_Plus.java:198)
 at ij.IJ.runUserPlugIn(IJ.java:217)
 at ij.IJ.runPlugIn(IJ.java:181)
 at ij.Executer.runCommand(Executer.java:137)
 at ij.Executer.run(Executer.java:66)
 at java.lang.Thread.run(Thread.java:662)
Caused by: java.lang.ClassNotFoundException: org.scijava.java3d.View
 at java.net.URLClassLoader$1.run(URLClassLoader.java:202)
 at java.security.AccessController.doPrivileged(Native Method)
 at java.net.URLClassLoader.findClass(URLClassLoader.java:190)
 at java.lang.ClassLoader.loadClass(ClassLoader.java:307)
 at sun.misc.Launcher$AppClassLoader.loadClass(Launcher.java:301)
 at java.lang.ClassLoader.loadClass(ClassLoader.java:248)
 ... 23 more

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