Posted by
Aryeh Weiss on
Jul 13, 2016; 11:22am
URL: http://imagej.273.s1.nabble.com/3D-viewing-of-a-z-stack-problem-with-channel-colors-tp5016866p5016867.html
On 13/07/2016 1:20 PM, emilia8 wrote:
> Hello everyone,
> so my problem is I'm trying to make a 3D image from images taken with a
> confocal microscope (.tiff images). I have all the files in the same folder,
> named "NAMEc1.tiff, NAMEc2.tiff etc" where c1 = blue images and c2 = red
> images. When I import the files (file -> import -> image sequences) all
> images become red (both blue c1 images and red c2 images).. why is this
> happening? if I separate the c1 files from c2 files the result is ok (blue
> is blue and red is red) but I can't do that with all my folders (some have
> 100+ files..).
When you import a sequence, you can specify a string that the filename
should contain. If you specify c1.tiff it will import only
the first channel, and c2.tiff will get you the second channel. Then it
will be easy to make them whatever color you want.
> Also another problem is it won't let me download the 3D viewer plugin..it
> says I don't have the permission to write to /Library/Java/Exstension .. I
> opened my home folder and created the Java/Extensions folder in the Library
> folder and changed the permission settings but it still doesn't work..
> I have a macbook pro!
I assume that it is trying to install Java 3D on you system. If you run
Fiji, then the 3D-viewer is available through the 3D update site. You
might see if that install what it needs without going to the
/Library/Java/Extensions
--aryeh
--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel
Ph: 972-3-5317638
FAX: 972-3-7384051
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