http://imagej.273.s1.nabble.com/Bug-in-Background-Subtracter-tp5016896p5016898.html
the nice 326x491 32bit image (what does it show?). You didn't mention
your setting of "Subtract background". I use all options unchecked or
> While finalising a new plugin I discovered a bug in Background Subtracter.
> In my own plugin I encounter a similar problem and do not know how to fix
> it. To reproduce the bug in Background Subtracter take the following steps:
>
> Bug in Background subtracter:
>
> 1: Load a tiff with three (double) channels
> 2: Image -> Color -> Split channels
> 3: for each slice (R, G and B): Image -> Type -> 32 bit
> 4: Image -> Color -> Merge channels
> 5: Process -> Subtract background
>
> You can also download this sample file from my dropbox:
>
>
https://www.dropbox.com/s/r0wbvft0xh6wucr/merge32-1.tif?dl=0>
> , load it into ImageJ and proceed with step 5.
>
> Now two of the three resulting channels will show black. They have sensible
> data but probably some min and max are off and so do not show properly.
>
> I noticed that by doing Image -> Adjust -> Window/Level -> Reset on the
> defective slice the image springs back and shows. I traced through the
> source of ij.plugin.frame.ContrastAdjuster.reset(ImagePlus, ImageProcessor)
> but did not succeed in taking similar steps in my own plugin to get the
> same result.
>
> Does anybody know how to reset a stack of 32-bit images to show properly
> after the values of the images have changed so much they fall outside their
> previous bounds? I suspect some series of ip.resetMinAndMax() but just that
> does not do.
>
> Greetings from Holland,
>
> Koen
>
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