http://imagej.273.s1.nabble.com/Bug-in-Background-Subtracter-tp5016896p5016914.html
However this is only affecting the display of your data. Take a close
look on Fijis main window as you hover over the image. It will tell
you the coordinates of your cursor and a value. That should tell
on screen. Simply go to brightnes&contrast and press the reset button for
> That is strange that you get expected results and I do not.
> What is also strange is when I run Background Subtracter I see a red image
> (only the red channel). When I save it and open it again I only see three
> black slices in this tiff.
>
> I am running ImageJ 1.151 on OSX 10.11.5.
>
> I tried again, and my resulting image is attached to this mail (hope that's
> possible through this listserver). A link to the resulting file on my
> dropbox is
>
>
https://www.dropbox.com/s/3ipgqn0johoyg8d/subtract%20background%20merge32-1.tif?dl=0>
> The image shows part of the (internal) female genitals of Coleophora
> flavipennella, a small micromoth.
>
> Koen
>
>
>
> On Sun, Jul 17, 2016 at 2:58 PM, Herbie <
[hidden email]> wrote:
>
>> Dear Koen van Dijken,
>>
>> I can't confirm your finding ("two of the three resulting channels will
>> show black"), when using ("load it into ImageJ and proceed with step 5")
>> the nice 326x491 32bit image (what does it show?). You didn't mention your
>> setting of "Subtract background". I use all options unchecked or "Light
>> background" checked and the default radius 50.
>>
>> Best
>>
>> Herbie
>>
>> :::::::::::::::::::::::::::::::::::::::::::::
>> Am 17.07.16 um 10:53 schrieb Koen van dijken:
>>
>>> While finalising a new plugin I discovered a bug in Background Subtracter.
>>> In my own plugin I encounter a similar problem and do not know how to fix
>>> it. To reproduce the bug in Background Subtracter take the following
>>> steps:
>>>
>>> Bug in Background subtracter:
>>>
>>> 1: Load a tiff with three (double) channels
>>> 2: Image -> Color -> Split channels
>>> 3: for each slice (R, G and B): Image -> Type -> 32 bit
>>> 4: Image -> Color -> Merge channels
>>> 5: Process -> Subtract background
>>>
>>> You can also download this sample file from my dropbox:
>>>
>>>
https://www.dropbox.com/s/r0wbvft0xh6wucr/merge32-1.tif?dl=0>>>
>>> , load it into ImageJ and proceed with step 5.
>>>
>>> Now two of the three resulting channels will show black. They have
>>> sensible
>>> data but probably some min and max are off and so do not show properly.
>>>
>>> I noticed that by doing Image -> Adjust -> Window/Level -> Reset on the
>>> defective slice the image springs back and shows. I traced through the
>>> source of ij.plugin.frame.ContrastAdjuster.reset(ImagePlus,
>>> ImageProcessor)
>>> but did not succeed in taking similar steps in my own plugin to get the
>>> same result.
>>>
>>> Does anybody know how to reset a stack of 32-bit images to show properly
>>> after the values of the images have changed so much they fall outside
>>> their
>>> previous bounds? I suspect some series of ip.resetMinAndMax() but just
>>> that
>>> does not do.
>>>
>>> Greetings from Holland,
>>>
>>> Koen
>>>
>>> --
>>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>>>
>>>
>> --
>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html