Extraction of slice having the maximum intensity in a specific channel
Posted by
cbenji59 on
Aug 01, 2016; 7:38am
URL: http://imagej.273.s1.nabble.com/Extraction-of-slice-having-the-maximum-intensity-in-a-specific-channel-tp5016959.html
Dear,
I am struggling with making a plugging in ImageJ / FiJi.
What I want to do, is from a TIFF file from a 13 stacks in Z, several point in time, and 4 channels, to create a new file (extraction) from the slice where there is more intensity.
Indeed, during my acquisition, I made a Z stack, I am only interested on having one slice, which is the more intense in the fluorescent channel.
Up-to-there, I am able to measure intensity in all the channel, all the time, and all the slice, but unable to from that to extract data in a way that I can ask the following:
"for each slice, which slice present the highest value in intensity"
Indeed, from that, I would be able to extract the given slice (for the 4 channels) and make a new file containing only the brightest slice for each time, based only on the value of the fluorescent channel (here channel 4 on the figure below).
Would it be possible to help me on this macro or maybe some have idea about an easiest way?
Many thanks by advance! :)