http://imagej.273.s1.nabble.com/Extraction-of-slice-having-the-maximum-intensity-in-a-specific-channel-tp5016959p5016962.html
> Dear,
>
> I am struggling with making a plugging in ImageJ / FiJi.
> What I want to do, is from a TIFF file from a 13 stacks in Z, several point
> in time, and 4 channels, to create a new file (extraction) from the slice
> where there is more intensity.
> Indeed, during my acquisition, I made a Z stack, I am only interested on
> having one slice, which is the more intense in the fluorescent channel.
>
> Up-to-there, I am able to measure intensity in all the channel, all the
> time, and all the slice, but unable to from that to extract data in a way
> that I can ask the following:
>
> "for each slice, which slice present the highest value in intensity"
>
> Indeed, from that, I would be able to extract the given slice (for the 4
> channels) and make a new file containing only the brightest slice for each
> time, based only on the value of the fluorescent channel (here channel 4 on
> the figure below).
>
> Would it be possible to help me on this macro or maybe some have idea about
> an easiest way?
> Many thanks by advance! :)
>
>
>
> <
http://imagej.1557.x6.nabble.com/file/n5016959/results.png>
>
>
>
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CEO: Dr. rer. nat. Jan Brocher