http://imagej.273.s1.nabble.com/From-3D-Projection-back-to-stack-tp5016974p5016978.html
Hopefully, the images are not 32bit...
> On Friday 05 Aug 2016 10:57:02 Herbie wrote:
>> Good day Romina Plitman,
>> in general it is impossible to reconstruct the constituents from their sum.
>>
>> The sum is e.g. 23, what are its 5 constituents?
>> This problem is ill-posed.
>
> Maybe there is kind of way round, or perhaps it is me no understanding the
> setup, apologies if so. Is the purpose to have the clean skeleton by cleaning
> the projection?
>
> You have the original stack, the projection and the cleaned projection.
>
> So you could get the projected version, subtract the cleaned projected version
> and you are left with the projection of all the bits that should be removed.
> Try subtracting this image from every slice in your volume.
>
> That being said, I do not think that this way of cleaning the stack is
> correct, because you cannot remove noise that is aligned in the z direction
> under" and "above" remaining components.
> Maybe you can minimise this a bit more by doing other orthogonal projections,
> not sure.
>
> Cheers
>
> Gabriel
>
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