ImageJ - Re: From 3D Projection back to stack

ImageJ

Re: From 3D Projection back to stack

Posted by Niko Ehrenfeuchter on Aug 05, 2016; 11:30am
URL: http://imagej.273.s1.nabble.com/From-3D-Projection-back-to-stack-tp5016974p5016979.html

I don't see a problem with 32 bit images, the skeleton is usually a
binarized version anyway that you could simply use as a mask on the raw
stack.

However, I agree with Gabriel that this is still a problem with
structures aligned in x/y...

Best,
Niko

On 05.08.2016 12:45, Herbie wrote:

> Dear Gabriel!
>
> "Try subtracting this image from every slice in your volume."
>
> Hopefully, the images are not 32bit...
>
> Best greetings
>
> Herbie
>
> :::::::::::::::::::::::::::::::::::::::::::::
> Am 05.08.16 um 12:29 schrieb Gabriel Landini:
>> On Friday 05 Aug 2016 10:57:02 Herbie wrote:
>>> Good day Romina Plitman,
>>> in general it is impossible to reconstruct the constituents from
>>> their sum.
>>>
>>> The sum is e.g. 23, what are its 5 constituents?
>>> This problem is ill-posed.
>>
>> Maybe there is kind of way round, or perhaps it is me no understanding
>> the
>> setup, apologies if so. Is the purpose to have the clean skeleton by
>> cleaning
>> the projection?
>>
>> You have the original stack, the projection and the cleaned projection.
>>
>> So you could get the projected version, subtract the cleaned projected
>> version
>> and you are left with the projection of all the bits that should be
>> removed.
>> Try subtracting this image from every slice in your volume.
>>
>> That being said, I do not think that this way of cleaning the stack is
>> correct, because you cannot remove noise that is aligned in the z
>> direction
>> under" and "above" remaining components.
>> Maybe you can minimise this a bit more by doing other orthogonal
>> projections,
>> not sure.
>>
>> Cheers
>>
>> Gabriel
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html