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Re: questions about grid stitching

Posted by Aryeh Weiss on Aug 11, 2016; 5:07am
URL: http://imagej.273.s1.nabble.com/questions-about-grid-stitching-tp5016991p5016999.html

HI Curtis

Thank you for your reply.

On 10/08/2016 10:14 PM, Curtis Rueden wrote:
> Hi Aryeh,
>
> > I would like the stitching to be done only in the xy directions, but
> not in Z.
>
> Did you try this?
> http://imagej.net/Image_Stitching#I_want_to_ignore_the_movement_of_tiles_in_Z
>
Perfect. Thank you for pointing this out.
> > Second - when I select "downsample tiles", the stitching does
> > something strange. It puts all of my grid of tiles in on row.
>
> Sounds like a bug. You can file an issue here:
> https://github.com/fiji/Stitching/issues
>
> It would be helpful to list the settings you used which produced the
> problem.
>
Done.
> Sorry to say I might not have time to investigate the issue right away.
>
No problem. Your efforts are greatly appreciated.

Best regards
--aryeh

> Regards,
> Curtis
>
> --
> Curtis Rueden
> LOCI software architect - http://loci.wisc.edu/software
> ImageJ2 lead, Fiji maintainer - http://imagej.net/User:Rueden
> Did you know ImageJ has a forum? http://forum.imagej.net/
>
>
> On Wed, Aug 10, 2016 at 1:54 PM, Aryeh Weiss <[hidden email]
> <mailto:[hidden email]>> wrote:
>
>     I have two questions about this plugin:
>
>     First - I have a set of XYZ stacks that I am stitching.
>
>     I would like the stitching to be done only in the xy directions,
>     but not in Z. That is, even if the stitcher thinks that a stack
>     should be shifted in Z, I do not want that to happen. I though
>     that selecting the "Ignore Z- stage position" would do this, but
>     it turns out that even with that option stacks are shifted in the
>     Z direction.
>
>     Is there a way to prevent this. I have the ROIs in the ROI
>     manager, but I do not want to write my own fusion program to fuse
>     the stacks.
>
>     Second - when I select "downsample tiles", the stitching does
>     something strange. It puts all of my grid of tiles in on row. When
>     I do nto down-sample, with all else the same, it works as
>     expected. Do I misunderstand what the downsample option does? I
>     was trying ot use it to same space on a really large stitched image.
>
>     Tnx in advance for your help,
>
>     --aryeh
>
>     --
>     Aryeh Weiss
>     Faculty of Engineering
>     Bar Ilan University
>     Ramat Gan 52900 Israel
>
>     Ph:  972-3-5317638
>     FAX: 972-3-7384051
>
>     --
>     ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>     <http://imagej.nih.gov/ij/list.html>
>
>


--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: 972-3-7384051


--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html