Segment phase contrast image

Posted by Laurent.guerard on Aug 17, 2016; 1:55pm
URL: http://imagej.273.s1.nabble.com/Segment-phase-contrast-image-tp5017034.html

Hi all,

I've been trying to segment a phase contrast image with no real success these past weeks.
The images are time series in order to track the movement of these cells. Would any of you have ideas on how to it ?

Here is an example :


There are 2 main problems with the images though :
1. There are dead cells/debris in the images which are very bright and might cause problems in the segmentation
2. The cells are really confluent, making it also difficult to separate close cells.

Any help would be welcome :)

Thanks.