> Hi all,
>
> I've been trying to segment a phase contrast image with no real success
> these past weeks.
> The images are time series in order to track the movement of these cells.
> Would any of you have ideas on how to it ?
>
> Here is an example :
> <http://imagej.1557.x6.nabble.com/file/n5017034/TiledImage_T00_Trans.png>
>
> There are 2 main problems with the images though :
> 1. There are dead cells/debris in the images which are very bright and might
> cause problems in the segmentation
> 2. The cells are really confluent, making it also difficult to separate
> close cells.
>
> Any help would be welcome :)
>
> Thanks.
>