Re: Using ImageJ for bacteriophage plaques
Posted by
Krs5 on
Aug 23, 2016; 7:46am
URL: http://imagej.273.s1.nabble.com/Using-ImageJ-for-bacteriophage-plaques-tp5017097p5017102.html
Dear Tamara,
You could try
selectWindow("LargePlaqueExample(July30th).png");
run("Median...", "radius=4");
run("Find Maxima...", "noise=8 output=[Maxima Within Tolerance] exclude light");
run("Analyze Particles...", "size=5-Infinity show=Nothing display summarize add");
selectWindow("LargePlaqueExample(July30th).png");
roiManager("Show All without labels");
You can change the radius of the Median filter (or use a different filter) and/or the noise setting in Find Maxima to optimize the results.
Good luck
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Tamara James
Sent: 22 August 2016 20:55
To:
[hidden email]
Subject: Using ImageJ for bacteriophage plaques
I have a question regarding an auto detection or manual selection of detected circles (bacteriophage Plaques)- I have attached the images here and any help would be terrific.
http://forum.imagej.net/t/image-analysis-for-bacteriophage-plaques/2549?u=tamarajameThank you
Tamara
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