Re: Using ImageJ for bacteriophage plaques

Posted by Jan Eglinger on Aug 23, 2016; 8:47am
URL: http://imagej.273.s1.nabble.com/Using-ImageJ-for-bacteriophage-plaques-tp5017097p5017103.html

Dear all,

please note that there also were several answers to the post on the
ImageJ forum. To allow others to follow, I'm posting the link again:

http://forum.imagej.net/t/image-analysis-for-bacteriophage-plaques/2549

Jan


On 23.08.2016 09:46, Straatman, Kees (Dr.) wrote:

> Dear Tamara,
>
> You could try
>
> selectWindow("LargePlaqueExample(July30th).png");
> run("Median...", "radius=4");
> run("Find Maxima...", "noise=8 output=[Maxima Within Tolerance] exclude light");
> run("Analyze Particles...", "size=5-Infinity show=Nothing display summarize add");
> selectWindow("LargePlaqueExample(July30th).png");
> roiManager("Show All without labels");
>
> You can change the radius of the Median filter (or use a different filter) and/or the noise setting in Find Maxima to optimize the results.
>
> Good luck
>
> Kees
>
>
> Dr Ir K.R. Straatman
> Senior Experimental Officer
> Advanced Imaging Facility
> Centre for Core Biotechnology Services
> University of Leicester
> http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif
>
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Tamara James
> Sent: 22 August 2016 20:55
> To: [hidden email]
> Subject: Using ImageJ for bacteriophage plaques
>
> I have a question regarding an auto detection or manual selection of detected circles (bacteriophage Plaques)- I have attached the images here and any help would be terrific.
>
> http://forum.imagej.net/t/image-analysis-for-bacteriophage-plaques/2549?u=tamarajame
>
> Thank you
> Tamara
>
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