Re: Making fluorescent intensity equivalent across images

Posted by samjlord on Aug 25, 2016; 5:28pm
URL: http://imagej.273.s1.nabble.com/Making-fluorescent-intensity-equivalent-across-images-tp5017113p5017119.html

jeslea wrote

Hi, I have a batch of fluorescent images which I would like to perform cell counts on. I noticed that the background intensity differs across images. In order for the counts to be comparable across images I would like to adjust all the images so that the level of background staining is equivalent. Is there a way to do this? Is there a way to adjust the intensity of the entire image until the background reaches a specified value which I will keep constant across images?

Under Image -> Adjust -> Brightness/Contrast, click "Set" and select "Propagate to all open images." Then all images will have the same display scaling.

-Sam