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Re: Making fluorescent intensity equivalent across images

Posted by GuyM on Aug 25, 2016; 6:24pm
URL: http://imagej.273.s1.nabble.com/Making-fluorescent-intensity-equivalent-across-images-tp5017113p5017120.html

Hi
You may find the "subtract background" command useful for your needs:

http://imagejdocu.tudor.lu/doku.php?id=gui:process:subtract_background

I think that it re-calculates the background for each slice, but even if not, implementing this in a macro should be quite simple.

Guy