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Re: Making fluorescent intensity equivalent across images

Posted by jerie on Aug 25, 2016; 6:26pm
URL: http://imagej.273.s1.nabble.com/Making-fluorescent-intensity-equivalent-across-images-tp5017113p5017121.html

Hi Jeslea,

Image -> Adjust -> bleach correction 'simple ratio' might do the job.

Abraços,Jens

Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
Brasil.

On Thu, Aug 25, 2016 at 3:52 AM, jeslea <[hidden email]> wrote:

> Hi, I have a batch of fluorescent images which I would like to perform cell
> counts on. I noticed that the background intensity differs across images.
> In
> order for the counts to be comparable across images I would like to adjust
> all the images so that the level of background staining is equivalent. Is
> there a way to do this? Is there a way to adjust the intensity of the
> entire
> image until the background reaches a specified value which I will keep
> constant across images?
>
> Thank you!
>
>
>
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Jens Rietdorf Visiting Scientist Fundação Oswaldo Cruz - Ministério da Saúde, Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Rio de Janeiro, Brasil.