Re: Scale is SOMETIMES lost during import

Posted by Herbie on Oct 10, 2016; 11:27am
URL: http://imagej.273.s1.nabble.com/Scale-is-SOMETIMES-lost-during-import-tp5017331p5017332.html

Dear Thorsten,

I can confirm your findings but is it really necessary to use such a huge stack for test purposes?

"unit is given in cm"

Yes, but this is a matter of taste. The values are correct anyway.

Best

Herbie

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Am 10.10.16 um 12:20 schrieb Thorsten Wagner:
> Dear List,
>
>
> I've encountered a problem when I try to import a set of images.
>
> To reproduce please download the following set of images:
>
> https://dl.dropboxusercontent.com/u/560426/TEST.zip (it's currently
> uploading)
>
> Now apply File -> Import -> Image Sequence and check "Use Virtual
> Stack". The data is correctly scaled to 0.66 x 0.66 µm.
>
> Now apply File -> Import -> Image Sequence again but do not check "Use
> Virtual Stack". Now the scale is lost.
>
> If you delete L'45AA7.tif the data will be correctly imported with the
> second approach as well.
>
> Did I found a bug?
>
> Another question:
> If you open a single image you will the that the unit is given in cm.
> But if you import it with the first approach, the unit is given in µm.
> Does ImageJ internally convert the units?
>
> Best,
> Thorsten