Cell automatic selection on bad image

Posted by Василий Попков on Oct 10, 2016; 12:38pm
URL: http://imagej.273.s1.nabble.com/Cell-automatic-selection-on-bad-image-tp5017334.html

Good time of a day, friends and colleagues.
Anyone can give me some advices how (and if - maybe it will be wiser not to
waste time) I can automaticly select cells (for roi manager) on such image?
For "eyes" it dosent look so bad, though yes, phase is awful, thankfully
fluorescent channel is ok, but it's hard to distinguish cells there. But I
have tried all more or less standart methods and they all were doing quite
bad selection.
Or, to be more cpecific, they select almost nothing - I'll be more or less
ok with "bad" selection if it will be bad selection of cells.
Otherwise I am going to manually select them with "cell magic wand tool".
Its just a bit "not clever" work.

Thanks,
Vasily

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Vasily A Popkov
Lab.Structure and function of mitochondria
A.N.Belozersky Institute of Physico-Chemical Biology
Moscow State University

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