Posted by
ctrueden on
Oct 10, 2016; 7:24pm
URL: http://imagej.273.s1.nabble.com/Cell-automatic-selection-on-bad-image-tp5017334p5017341.html
Hi Vasily,
Did you try the Trainable Weka Segmentation?
http://imagej.net/Trainable_Weka_Segmentationhttp://forum.imagej.net/t/analysis-of-aggregates/303/3?u=ctruedenhttp://imagej.net/SegmentationNote the following helpful comment in that forum thread: "In the settings,
I had added the Variance and Structure features, increased the maximum
sigma to 32, and checked Homogenize classes"
I did not have time right now to try it with your data, but perhaps it
helps you.
Regards,
Curtis
--
Curtis Rueden
LOCI software architect -
http://loci.wisc.edu/softwareImageJ2 lead, Fiji maintainer -
http://imagej.net/User:RuedenDid you know ImageJ has a forum?
http://forum.imagej.net/On Mon, Oct 10, 2016 at 7:38 AM, Василий Попков <
[hidden email]>
wrote:
> Good time of a day, friends and colleagues.
> Anyone can give me some advices how (and if - maybe it will be wiser not to
> waste time) I can automaticly select cells (for roi manager) on such image?
> For "eyes" it dosent look so bad, though yes, phase is awful, thankfully
> fluorescent channel is ok, but it's hard to distinguish cells there. But I
> have tried all more or less standart methods and they all were doing quite
> bad selection.
> Or, to be more cpecific, they select almost nothing - I'll be more or less
> ok with "bad" selection if it will be bad selection of cells.
> Otherwise I am going to manually select them with "cell magic wand tool".
> Its just a bit "not clever" work.
>
> Thanks,
> Vasily
>
> --
> Vasily A Popkov
> Lab.Structure and function of mitochondria
> A.N.Belozersky Institute of Physico-Chemical Biology
> Moscow State University
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html