http://imagej.273.s1.nabble.com/Cell-automatic-selection-on-bad-image-tp5017334p5017344.html
Yes, I have tried it, and it didn't work well. Though I have little
experience with weka. So, since you are advising it, I'll check some
manuals and will try more. Without some expert opinion it is hard to
> Hi Vasily,
>
> Did you try the Trainable Weka Segmentation?
>
>
http://imagej.net/Trainable_Weka_Segmentation>
http://forum.imagej.net/t/analysis-of-aggregates/303/3?u=ctrueden>
http://imagej.net/Segmentation>
> Note the following helpful comment in that forum thread: "In the settings,
> I had added the Variance and Structure features, increased the maximum
> sigma to 32, and checked Homogenize classes"
>
> I did not have time right now to try it with your data, but perhaps it
> helps you.
>
> Regards,
> Curtis
>
> --
> Curtis Rueden
> LOCI software architect -
http://loci.wisc.edu/software> ImageJ2 lead, Fiji maintainer -
http://imagej.net/User:Rueden> Did you know ImageJ has a forum?
http://forum.imagej.net/>
>
> On Mon, Oct 10, 2016 at 7:38 AM, Василий Попков <
[hidden email]>
> wrote:
>
> > Good time of a day, friends and colleagues.
> > Anyone can give me some advices how (and if - maybe it will be wiser not
> to
> > waste time) I can automaticly select cells (for roi manager) on such
> image?
> > For "eyes" it dosent look so bad, though yes, phase is awful, thankfully
> > fluorescent channel is ok, but it's hard to distinguish cells there. But
> I
> > have tried all more or less standart methods and they all were doing
> quite
> > bad selection.
> > Or, to be more cpecific, they select almost nothing - I'll be more or
> less
> > ok with "bad" selection if it will be bad selection of cells.
> > Otherwise I am going to manually select them with "cell magic wand tool".
> > Its just a bit "not clever" work.
> >
> > Thanks,
> > Vasily
> >
> > --
> > Vasily A Popkov
> > Lab.Structure and function of mitochondria
> > A.N.Belozersky Institute of Physico-Chemical Biology
> > Moscow State University
> >
> > --
> > ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> >
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>