Posted by
PEARSON Matthew on
URL: http://imagej.273.s1.nabble.com/Image-ID-increment-value-when-image-cropped-tp5017411p5017416.html
Hi Michael,
Something is a bit odd as i wrote my own version of your little test macro below, to try to demonstrate the fault but it worked fine. However, if you follow these exact steps outside of a macro and use Image>Show Info as the means to report the imageID you'll see that the "red" plane of the clown image has the id of the unspilt crop - 2 not -1.
Interested to know if you find the same.
Thanks,
Matt
run("Clown (14K)");
original = getImageID();
print(original);
makeRectangle(114, 66, 50, 38);
run("Crop");
crop = getImageID();
print(crop);
Run("Split Channels");
selectImage(crop-1);
red = getImageID();
print(red);
--
Matt Pearson
Microscopy Facility
MRC Human Genetics Unit
Institute of Genetics and Molecular Medicine (IGMM)
University of Edinburgh
Crewe Road
EH4 2XU
On 19 Oct 2016, at 10:12, Michael Schmid <
[hidden email]<mailto:
[hidden email]>>
wrote:
Hi Matt,
sorry, I can't reproduce the problem. Try the following macro:
//run("HeLa Cells (1.3M, 48-bit RGB)");
run("Clown (14K)");
getImageID();
makeRectangle(114, 66, 50, 38);
run("Crop");
getImageID();
run("Split Channels");
//selectWindow("C1-hela-cells.tif");
selectWindow("clown.jpg (red)");
getImageID();
It's the same for the 'Clown' (RGB) and 'HeLa Cells' (Composite) images, the first image after splitting gets the first free ID, i.e., the ID of the image just opened before minus one.
Of course, if there is some step creating an ImagePlus between opening the original image and 'Split Channels', the imageID of the result will be lower.
So, if you cannot exclude that an image will be generated in between, it would be better to select the results of the 'split' operation by the new window title, which is the old title plus " (red)" etc. for RGB images, and "C"+the channel number (starting with 1)+"-"+the title for composite images.
Michael
________________________________________________________________
On 18 Oct 2016, at 15:43, PEARSON Matthew wrote:
Hi all,
I was wondering if any one knows why when you split channels on a
cropped image the ID number of the first split channel is 1st channel
ID = cropped image ID - 2. If i didn't crop the image the first
split channel has ID original - 1.
This is having some implications for me in a macro whereby ideally
the user can run it on the whole image or crop to an ROI first.
However my loop to determine channel id's of windows once it's split
channels is messed up if they crop first as the ID of the first
channel jumps -2 instead of -1!
I'm sure this is probably intentional behaviour and if so i will
create some extra lines to deal with this potential change.
Thanks for the help,
Matt
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