Re: question on image J

Posted by Anne Chetty on Oct 19, 2016; 3:20pm
URL: http://imagej.273.s1.nabble.com/question-on-image-J-tp5017405p5017425.html

Hi Ignacio,

I do somewhat similar measurements for Western blots. For immunostain when
you use the same,  do you have to go to edit, invert first and then do
measurements?

2. How do i keep the same area to measure different intensities in
different figures.  Appreciate your help.

Thanks,
 Anne

Anne

On Wed, Oct 19, 2016 at 10:45 AM, Ignacio Arganda-Carreras <
[hidden email]> wrote:

> Hello Anne,
>
> You need to delineate your regions of interest using a selection tool and
> then click on Analyze > Measure.
>
> Have a look at the manual entry for the Analyze menu:
> http://imagej.net/ij/docs/guide/146-30.html
>
> Cheers!
>
> ignacio
>
> On Mon, Oct 17, 2016 at 7:03 PM, Anne Chetty <[hidden email]> wrote:
>
> > I am trying to measure immunohistochemistry stains in a lung specimens.
> > How do i measure the area and or intensity of certain stains using
> imageJ?
> >
> > Thanks, Anne
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
>
>
> --
> Ignacio Arganda-Carreras, Ph.D.
> Ikerbasque Research Fellow
> Departamento de Ciencia de la Computacion e Inteligencia Artificial
> Facultad de Informatica, Universidad del Pais Vasco
> Paseo de Manuel Lardizabal, 1
> 20018 Donostia-San Sebastian
> Guipuzcoa, Spain
>
> Phone : [phone]
> Website: http://sites.google.com/site/iargandacarreras/
> <http://biocomp.cnb.csic.es/~iarganda/index_EN.html>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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