Re: question on image J

Posted by Ignacio Arganda-Carreras-2 on Oct 20, 2016; 7:45am
URL: http://imagej.273.s1.nabble.com/question-on-image-J-tp5017405p5017427.html

Hello Anne,


I do somewhat similar measurements for Western blots. For immunostain when
> you use the same,  do you have to go to edit, invert first and then do
> measurements?
>

If you want to measure the real intensity values, you shouldn't invert the
image (unless it was already inverted).


>
> 2. How do i keep the same area to measure different intensities in
> different figures.  Appreciate your help.
>

For that, ImageJ has the ROI Manager
<http://imagej.net/ij/docs/guide/146-30.html#sub:ROI-Manager...>, where you
can store the selections you made on one image and use them later on
another image.

Cheers!

PS: I recommend you to post your questions in the ImageJ forum
<http://forum.imagej.net> in the future as well.

>
> Thanks,
>  Anne
>
> Anne
>
> On Wed, Oct 19, 2016 at 10:45 AM, Ignacio Arganda-Carreras <
> [hidden email]> wrote:
>
> > Hello Anne,
> >
> > You need to delineate your regions of interest using a selection tool and
> > then click on Analyze > Measure.
> >
> > Have a look at the manual entry for the Analyze menu:
> > http://imagej.net/ij/docs/guide/146-30.html
> >
> > Cheers!
> >
> > ignacio
> >
> > On Mon, Oct 17, 2016 at 7:03 PM, Anne Chetty <[hidden email]> wrote:
> >
> > > I am trying to measure immunohistochemistry stains in a lung specimens.
> > > How do i measure the area and or intensity of certain stains using
> > imageJ?
> > >
> > > Thanks, Anne
> > >
> > > --
> > > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> > >
> >
> >
> >
> > --
> > Ignacio Arganda-Carreras, Ph.D.
> > Ikerbasque Research Fellow
> > Departamento de Ciencia de la Computacion e Inteligencia Artificial
> > Facultad de Informatica, Universidad del Pais Vasco
> > Paseo de Manuel Lardizabal, 1
> > 20018 Donostia-San Sebastian
> > Guipuzcoa, Spain
> >
> > Phone : [phone]
> > Website: http://sites.google.com/site/iargandacarreras/
> > <http://biocomp.cnb.csic.es/~iarganda/index_EN.html>
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
Ignacio Arganda-Carreras, Ph.D.
Ikerbasque Research Fellow
Departamento de Ciencia de la Computacion e Inteligencia Artificial
Facultad de Informatica, Universidad del Pais Vasco
Paseo de Manuel Lardizabal, 1
20018 Donostia-San Sebastian
Guipuzcoa, Spain

Phone : [phone]
Website: http://sites.google.com/site/iargandacarreras/
<http://biocomp.cnb.csic.es/~iarganda/index_EN.html>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html