Re: question on image J
Posted by
Gabriel Landini on
Oct 20, 2016; 7:58am
URL: http://imagej.273.s1.nabble.com/question-on-image-J-tp5017405p5017428.html
On Wednesday 19 Oct 2016 11:20:21 Anne Chetty wrote:
> I do somewhat similar measurements for Western blots. For immunostain when
> you use the same, do you have to go to edit, invert first and then do
> measurements?
>
> 2. How do i keep the same area to measure different intensities in
> different figures. Appreciate your help.
Please be aware that measuring "immunostain intensity" is not a sound approach
to quantify antigen expression.
Please see here:
https://list.nih.gov/cgi-bin/wa.exe?A2=ind0902&L=IMAGEJ&P=R18412Regards
Gabriel
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