Re: Scale Bar LSM format image

Posted by Sindy Kueh-2 on Oct 21, 2016; 1:11am
URL: http://imagej.273.s1.nabble.com/Re-Scale-Bar-LSM-format-image-tp5017439.html

Dear Image J users

I would like to add scale bar to images captured using the ZEN 2009 software. Images are store as LSM files. Unfortunately, the output scale seemed to be incorrect.
I have attached a picture of muscle fibres, showing the scale bar on bottom left which is incorrect: each fibre diameter is estimated to be in the range of 20-40uM, Do I need to calibrate Fiji - pixel to uM?

Thank you
Sindy



Sindy Kueh, PhD| Research Officer
Anatomy and Cell Biology|School of Medicine

T: 02-4620 3938|  F: 02 4620 3890| E:  [hidden email]

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