Re: Scale is SOMETIMES lost during import

Posted by Rasband, Wayne (NIH/NIMH) [E] on Oct 22, 2016; 11:48pm
URL: http://imagej.273.s1.nabble.com/Scale-is-SOMETIMES-lost-during-import-tp5017331p5017450.html

> On Oct 10, 2016, at 6:20 AM, Thorsten Wagner <[hidden email]> wrote:
>
> Dear List,
>
> I've encountered a problem when I try to import a set of images.
>
> To reproduce please download the following set of images:
>
> https://dl.dropboxusercontent.com/u/560426/TEST.zip (it's currently
> uploading)
>
> Now apply File -> Import -> Image Sequence and check "Use Virtual
> Stack". The data is correctly scaled to 0.66 x 0.66 µm.
>
> Now apply File -> Import -> Image Sequence again but do not check "Use
> Virtual Stack". Now the scale is lost.
>
> If you delete L'45AA7.tif the data will be correctly imported with the
> second approach as well.
>
> Did I found a bug?

This bug is fixed in the latest ImageJ daily build (1.51h12).

> Another question:
> If you open a single image you will the that the unit is given in cm.
> But if you import it with the first approach, the unit is given in µm.
> Does ImageJ internally convert the units?

Units are converted from cm to µm when the pixel width is less than 0.0001 cm. Earlier versions of ImageJ only did this conversion when importing a sequence of images as a virtual stack.

-wayne


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