Re: grey scale skeleton transform

Posted by Herbie on Oct 31, 2016; 10:03am
URL: http://imagej.273.s1.nabble.com/grey-scale-skeleton-transform-tp5017505p5017506.html

Good day Joost,

an typical image would be helpful...

Did you try to highpass filter the image?

Best

Herbie

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Am 31.10.16 um 10:51 schrieb Joost Willemse:

> Hi everyone,
>
> Does anyone know a plugin to create a skeletonized image based on a
> grey-scale images instead of based on binary images. I have pictures which
> have an intense line of fluorescence which I want to localize precisely but
> there is quite some variation along the length of the line which makes a
> normal thresholding insufficient.
>
> If i do skeletonize the binary image I get the middle of my region back
> which does not necessarily coincide with the highest intensity skeleton
> from the region.
>
> All help is appreciated
> Joost willemse
> Leiden University
>
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>

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