Posted by
Straub, Volko A. (Dr.) on
Oct 31, 2016; 1:00pm
URL: http://imagej.273.s1.nabble.com/grey-scale-skeleton-transform-tp5017505p5017508.html
Hi Joost,
You could try applying a 'tubeness filter' to the image prior to thresholding (try Fiji -> Plugins -> Analyze -> Tubeness). This can help to separate lines of variable intensity from background. Alternatively, a local thresholding method might be useful.
As Herbie said, a sample image would be useful.
Volko
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Herbie
Sent: 31 October 2016 10:04
To:
[hidden email]
Subject: Re: grey scale skeleton transform
Good day Joost,
an typical image would be helpful...
Did you try to highpass filter the image?
Best
Herbie
::::::::::::::::::::::::::::::::::::::::::::
Am 31.10.16 um 10:51 schrieb Joost Willemse:
> Hi everyone,
>
> Does anyone know a plugin to create a skeletonized image based on a
> grey-scale images instead of based on binary images. I have pictures
> which have an intense line of fluorescence which I want to localize
> precisely but there is quite some variation along the length of the
> line which makes a normal thresholding insufficient.
>
> If i do skeletonize the binary image I get the middle of my region
> back which does not necessarily coincide with the highest intensity
> skeleton from the region.
>
> All help is appreciated
> Joost willemse
> Leiden University
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html