Posted by
Ignacio Arganda-Carreras-2 on
Nov 14, 2016; 10:01am
URL: http://imagej.273.s1.nabble.com/Local-Background-Subtraction-tp5017573p5017574.html
Dear Joel,
That is similar to applying a White Top Hat filter with a large structuring
element. Have a look at that morphological filter option in MorphoLibJ
<
http://imagej.net/MorphoLibJ#Top-hats>.
Cheers!
ignacio
On Sun, Nov 13, 2016 at 6:13 PM, JOEL B. SHEFFIELD <
[hidden email]> wrote:
> Greetings all,
>
> We are interested in quantitating the intensity of spots in dot blot
> experiments in which the background is uneven. Some of the commercial
> software packages have instituted a routine that essentially thresholds
> each spot, dilates the area by a fixed amount, calculates the per pixel
> density in the expanded region, and then subtracts that value from each
> pixel in the sample area.
>
> A quick scan through the mailing list, as well as the plugins list, didn't
> come up with anything.
>
> Is anyone aware of such a plugin/macro for IJ?
>
> Joel
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail:
[hidden email]
> URL: *
http://tinyurl.com/khbouft <
http://tinyurl.com/khbouft>*
>
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http://imagej.nih.gov/ij/list.html>
--
Ignacio Arganda-Carreras, Ph.D.
Ikerbasque Research Fellow
Departamento de Ciencia de la Computacion e Inteligencia Artificial
Facultad de Informatica, Universidad del Pais Vasco
Paseo de Manuel Lardizabal, 1
20018 Donostia-San Sebastian
Guipuzcoa, Spain
Phone : +34 943 01 73 25
Website:
http://sites.google.com/site/iargandacarreras/<
http://biocomp.cnb.csic.es/~iarganda/index_EN.html>
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