Re: Local Background Subtraction

Posted by Gabriel Landini on Nov 14, 2016; 10:27am
URL: http://imagej.273.s1.nabble.com/Local-Background-Subtraction-tp5017573p5017575.html

On Sunday 13 Nov 2016 12:13:43 JOEL B. SHEFFIELD wrote:
> We are interested in quantitating the intensity of spots in dot blot
> experiments in which the background is uneven.  Some of the commercial
> software packages have instituted a routine that essentially thresholds
> each spot, dilates the area by a fixed amount, calculates the per pixel
> density in the expanded region, and then subtracts that value from each
> pixel in the sample area.

Hi Joel,  Are your spots all the same size? (like the dot-blot sample image?).

Regards

Gabriel

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