Re: Local Background Subtraction

Posted by Ignacio Arganda-Carreras-2 on Nov 14, 2016; 2:20pm
URL: http://imagej.273.s1.nabble.com/Local-Background-Subtraction-tp5017573p5017577.html

Hello again, Joel,

If you post an image here or in the ImageJ forum <http://forum.imagej.net/>,
we can advice you on the best method available in ImageJ to solve your
problem.

ignacio

On Mon, Nov 14, 2016 at 2:34 PM, JOEL B. SHEFFIELD <[hidden email]> wrote:

> Thanks Ignacio and Gabriel,
>
> I'll try the tophat filter.  Meanwhile, for Gabriel, the spots can vary in
> size.  I was also thinking of this as another approach to gel analysis, and
> so I was hoping for some flexibility in defining the shape.
>
> Joel
>
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail: [hidden email]
> URL:  *http://tinyurl.com/khbouft <http://tinyurl.com/khbouft>*
>
> On Mon, Nov 14, 2016 at 5:27 AM, Gabriel Landini <[hidden email]>
> wrote:
>
> > On Sunday 13 Nov 2016 12:13:43 JOEL B. SHEFFIELD wrote:
> > > We are interested in quantitating the intensity of spots in dot blot
> > > experiments in which the background is uneven.  Some of the commercial
> > > software packages have instituted a routine that essentially thresholds
> > > each spot, dilates the area by a fixed amount, calculates the per pixel
> > > density in the expanded region, and then subtracts that value from each
> > > pixel in the sample area.
> >
> > Hi Joel,  Are your spots all the same size? (like the dot-blot sample
> > image?).
> >
> > Regards
> >
> > Gabriel
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
Ignacio Arganda-Carreras, Ph.D.
Ikerbasque Research Fellow
Departamento de Ciencia de la Computacion e Inteligencia Artificial
Facultad de Informatica, Universidad del Pais Vasco
Paseo de Manuel Lardizabal, 1
20018 Donostia-San Sebastian
Guipuzcoa, Spain

Phone : [phone]
Website: http://sites.google.com/site/iargandacarreras/
<http://biocomp.cnb.csic.es/~iarganda/index_EN.html>

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