Create Mask from blurred ROI

Posted by MattLee on Nov 18, 2016; 4:02pm
URL: http://imagej.273.s1.nabble.com/Create-Mask-from-blurred-ROI-tp5017598.html

Hello,

I'm looking to create distinct ROI's from the attached image using
intensity. From the image it is clear there are 3 cells in the centre
however when I create a Mask it combines these into a single ROI. Altering
the threshold doesn't really help either.

Is there a way to outline each cell using the intensity drop of the pixels
as a sort of Watershed?  The Watershed process requires a binary image, but
converting this image to binary combines them, so is not very helpful.

Thanks.

ROI


Mask