Chromatic shift correction

Posted by Rafael Buono on Nov 28, 2016; 8:08pm
URL: http://imagej.273.s1.nabble.com/Chromatic-shift-correction-tp5017645.html

Dear list,

I am working on a dual camera spinning disk system and would like to use ImageJ to correct for chromatic shift between the two channels (the two cameras).
I have data from fluorescent beads and would like to use this data to calculate the shifts (and potential warping) between the channels and apply the necessary transformations to my data (dual channel, z-stack, time course datasets). Is there a plugin that can do this?

I have read the entry on imagej.net ( http://imagej.net/Chromatic_shift_origins_measurement_and_correction ), but I have not found an automated way to get ImageJ to use the bead data to apply corrections to my actual data. How are you handling this type of problem (having to correct for chromatic shifts between channels)?

Best,

Rafael Buono

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