Posted by
Michael Schmid on
Nov 30, 2016; 7:28pm
URL: http://imagej.273.s1.nabble.com/stack-combiner-behaving-strangely-tp5017660p5017666.html
Hi Tim,
sorry, I can't reproduce this. Try this macro:
run("HeLa Cells (1.3M, 48-bit RGB)");
makeRectangle(59, 66, 114, 114);
run("Duplicate...", "title=dup-1 duplicate");
selectWindow("hela-cells.tif");
makeRectangle(275, 82, 114, 114);
run("Duplicate...", "title=dup-2 duplicate");
selectWindow("hela-cells.tif");
makeRectangle(367, 329, 114, 114);
run("Crop");
run("Combine...", "stack1=hela-cells.tif stack2=dup-1");
run("Combine...", "stack1=[Combined Stacks] stack2=dup-2");
run("Select All");
I also tried all three possibilities which 'axis' to define for the
three stack slices in Image>Properties<: c, z, t; it works for all.
I am using a recent daily build (plain ImageJ, not Fiji):
ImageJ 1.51i7; Java 1.6.0_65 [64-bit]
Michael
________________________________________________________________
On 2016-11-30 19:35, Feinstein, Timothy N wrote:
> Updating my last message, it appears that none of the 100x100 images
> created by the Duplicate command (cmd+shift+D, all 3 channels) are
> compatible with each other. Every pairwise combination leads to an error
> message that they are not the same data type. I cannot see anything about
> the images themselves that would make the computer think this.
>
>
> Tim
>
> Timothy Feinstein, Ph.D.
> Research Scientist
> University of Pittsburgh Department of Developmental Biology
>
>
>
>
>
> On 11/30/16, 1:24 PM, "Feinstein, Timothy N" <
[hidden email]> wrote:
>
>> Hello,
>>
>> I have noticed an odd behavior in the Image>Stacks>Tools>CombineŠ command.
>> Say that I have several 100x100 ROIs in a large 16 bit 3-channel image
>> that I want to stitch into a mosaic while discarding the rest of the
>> image. I create a 100x100 rectangle, duplicate out a few ROIs and use the
>> crop command to make the last 100x100 ROI. As far as I can tell the
>> duplicated ROIs and the cropped ROI are identical. Two duplicated ROIs
>> will combine with no problem but when I try to combine a duplicated ROI
>> with a cropped ROI it tells me that they are not the same data type. I am
>> using the latest Fiji update in OSX 10.10.5. Can anyone replicate this?
>>
>> Thanks,
>>
>>
>> Tim
>>
>> Timothy Feinstein, Ph.D.
>> Research Scientist
>> University of Pittsburgh Department of Developmental Biology
>>
>
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