Posted by
Pablo Munoz-2 on
Dec 06, 2016; 5:59pm
URL: http://imagej.273.s1.nabble.com/Help-with-macro-tp5017698.html
Dear ImageJ community,
I want to create a macro to analyse a complete folder of my images since
I do not know how to write the macro I am using the macro record tool.
This is the macro with my instructions,
selectWindow("1 control.lsm");
run("Subtract Background...", "rolling=300 stack");
run("Median...", "radius=1.2 stack");
run("StackReg", "transformation=Translation disable_global");
run("Split Channels");
selectWindow("C1-1 control.lsm");
run("Duplicate...", "title=CFP");
setAutoThreshold("Default dark");
//run("Threshold...");
setOption("BlackBackground", false);
run("Convert to Mask");
run("Divide...", "value=255.000");
imageCalculator("Divide create 32-bit", "C2-1 control.lsm","C1-1
control.lsm");
selectWindow("Result of C2-1 control.lsm");
imageCalculator("Divide create 32-bit", "Result of C2-1 control.lsm","CFP");
selectWindow("Result of Result of C2-1 control.lsm");
run("16 colors");
run("Scale Bar...", "width=30 height=8 font=28 color=White background=None
location=[Lower Right] bold");
run("Calibration Bar...", "location=[Upper Right] fill=White label=Black
number=5 decimal=1 font=12 zoom=2");
My problem is when I try to do this with another image with differents name
(e.g 1 Treatment.lsm, 2 Treatment.lsm , etc) the macro does not work.
I would like to do this process to a complete folder of images which the
name of the images are different and save the result in another folder.
Furthermore, I would like that the macro asks me to adjust the Threshold.
Any suggestion and help I will really appreciate and will save me a lot of
hour behind the computer.
Thanks in advance.
Pablo M.
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