> Dear Pablo,
>
> a viable option to apply recorded macros to a number of files in a set
> directory is by using
http://imagej.net/Batch_Processing.
>
> Hope that helps,
>
> Abraços, Jens
>
> Dr. Jens Rietdorf, visiting scientist @ center for technological
> development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
> Brasil.
>
> On Tue, Dec 6, 2016 at 3:59 PM, Pablo M <
[hidden email]> wrote:
>
> > Dear ImageJ community,
> >
> > I want to create a macro to analyse a complete folder of my images
> since
> > I do not know how to write the macro I am using the macro record tool.
> >
> > This is the macro with my instructions,
> >
> > selectWindow("1 control.lsm");
> > run("Subtract Background...", "rolling=300 stack");
> > run("Median...", "radius=1.2 stack");
> > run("StackReg", "transformation=Translation disable_global");
> > run("Split Channels");
> > selectWindow("C1-1 control.lsm");
> > run("Duplicate...", "title=CFP");
> > setAutoThreshold("Default dark");
> > //run("Threshold...");
> > setOption("BlackBackground", false);
> > run("Convert to Mask");
> > run("Divide...", "value=255.000");
> > imageCalculator("Divide create 32-bit", "C2-1 control.lsm","C1-1
> > control.lsm");
> > selectWindow("Result of C2-1 control.lsm");
> > imageCalculator("Divide create 32-bit", "Result of C2-1
> > control.lsm","CFP");
> > selectWindow("Result of Result of C2-1 control.lsm");
> > run("16 colors");
> > run("Scale Bar...", "width=30 height=8 font=28 color=White
> background=None
> > location=[Lower Right] bold");
> > run("Calibration Bar...", "location=[Upper Right] fill=White label=Black
> > number=5 decimal=1 font=12 zoom=2");
> >
> > My problem is when I try to do this with another image with differents
> name
> > (e.g 1 Treatment.lsm, 2 Treatment.lsm , etc) the macro does not work.
> > I would like to do this process to a complete folder of images which the
> > name of the images are different and save the result in another folder.
> >
> > Furthermore, I would like that the macro asks me to adjust the
> Threshold.
> >
> > Any suggestion and help I will really appreciate and will save me a lot
> of
> > hour behind the computer.
> >
> > Thanks in advance.
> >
> > Pablo M.
> >
> > --
> > ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> >
>
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