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Re: Stitching output has missing pixels

Posted by Aryeh Weiss on Dec 08, 2016; 7:14pm
URL: http://imagej.273.s1.nabble.com/Stitching-output-has-missing-pixels-tp5017714p5017724.html

I posted on this issue , most recently:
https://list.nih.gov/cgi-bin/wa.exe?A2=ind1610&L=IMAGEJ&F=&S=&P=41767

I characterized what happens as the stitching is done over larger areas.

By looking at the tiles near the blank area, you can get a decent
estimate of where to put the missing tiles. However, it would be nice to
not have to do that.

Best regards
--aryeh

On 08/12/2016 16:59, Majurski, Michael Paul (Fed) wrote:

> Mike,
>
> I have run into that missing output tile bug myself a few times and so far I have not seen neither a fix nor speculation as to the cause.
>
> At times I have had the first few tiles end up missing from the output image when I know I am using the plugin correctly. So your bug 1 is not a user error. However, I have never has small blocks of the output image go missing (bug 2 in your email) that were not in the upper left corner (tile 1, tile 1-2). So I cannot comment on that apparent bug.
>
> Another resource (if you have not found it already) would be the Fiji forum: http://forum.imagej.net/,  where there have been a few discussion related to Stephan's stitching plugin.
>
> However, there are other stitching programs to try which might result in less effort for you. The one I was involved in creating (MIST https://pages.nist.gov/MIST/ ) requires a regular grid of images (basically roughly constant overlap), but it might be worth giving it a try.
>
> Project Page: https://pages.nist.gov/MIST/
> Source: https://github.com/usnistgov/MIST
> Wiki: https://github.com/usnistgov/MIST/wiki
> Install Guide: https://github.com/usnistgov/MIST/wiki/Install-Guide
>
> Best of luck,
> Michael Majurski
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of MChapman
> Sent: Wednesday, December 07, 2016 17:14
> To: [hidden email]
> Subject: Stitching output has missing pixels
>
> Hello,
>
> I have been fighting Stephan's stitching plugin for a few months now and I just wanted to let you guys know what my issues have been if there is a hope at solving them.  I really like the stitching algorithm, there are just two errors that I have been experiencing.
>
> 1) When stitching more than about 50-100 images, the first or first and second tile don't find an adequate alignment and are left out of the stitch and assigned a position of (0,0) in the  TileConfiguration.txt file.
>
> To correct this, I just run a second stitch of the first 2-3 rows of the montage.  Then I use the position of an image in the middle of the bottom row of this stitch as a reference and rewrite the TileConfiguration.txt file positions for the full stitch.  I can then rerun the stitching program without "compute overlap" with the repaired TileConfiguration.txt and this fixes the first error.  A bit of a nuisance, but it works.
>
> 2) When I rerun the stitches with the repaired TileConfiguration.txt, some of the pixels (sometimes a single pixel, sometimes a block of 20x300) come back as 0 in the stitch.  There are no gaps in the overlap of the images from the TileConfiguration.txt file, and if I rerun the stitch again the 0 pixels show up in different locations.
>
> My current fix is to just run multiple restitches and then take a max of a stack of the stitches and do that until there are no 0 pixels.  Again this is a nuisance but I can get my data stitched with it.
>
> Is there anything that I am doing wrong or is anybody else having these issues?
>
> Mike
>
> edit* PS...If example images would be helpful, just let me know and I can post them or post a link to them.
> I reposted this correctly this time to send it out to the mailing list MIke
>
>
>
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>
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--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: 972-3-7384051

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