Re: Stitching output has missing pixels

Posted by MChapman on Dec 09, 2016; 3:47pm
URL: http://imagej.273.s1.nabble.com/Stitching-output-has-missing-pixels-tp5017714p5017737.html

Curtis,

I would be happy to share, and I have been trying to figure out how to upload these two images (16bit, 1292x968 .tif) but whenever I try to shrink the images or change the file type this stitching error doesn't occur anymore.  I also need to get these images cleared for release which may take a few weeks to a month.

If there is somewhere that I could post the pictures in their raw form I will.

The macro for the stitch is:
        run("Grid/Collection stitching", "type=[Grid: snake by rows] order=[Right & Down                ] grid_size_x=2 grid_size_y=1 tile_overlap=20 first_file_index_i=0 directory=[E:\ImageJ mailing list] file_names=1000{i}.tif output_textfile_name=TileConfiguration.txt fusion_method=[Linear Blending] regression_threshold=0.30 max/avg_displacement_threshold=2.50 absolute_displacement_threshold=3.50 compute_overlap subpixel_accuracy computation_parameters=[Save computation time (but use more RAM)] image_output=[Fuse and display]");

and the resulting log file output is:
Stitching internal version: 1.2
Loading (0, 0): E:\\ImageJ mailing list\10000.tif ...
1292x968px, channels=1, timepoints=1 (80 ms)
Loading (1, 0): E:\\ImageJ mailing list\10001.tif ...
1292x968px, channels=1, timepoints=1 (70 ms)
10000.tif[1] <- 10001.tif[1]: (1037.8536, -1.7621746) correlation (R)=0.29953933 (150 ms)
ERROR: Error: No correlated tiles found, setting the first tile to (0, 0).
 number of tiles = 1
Finished registration process (150 ms).
10000.tif: [3,3](AffineTransform[[1.0, 0.0, 0.0], [0.0, 1.0, 0.0]]) 1.7976931348623157E308
Writing registered TileConfiguration: E:ImageJ mailing list\TileConfiguration.registered.txt
Fuse & Display ...
Finished fusion (70 ms)
Finished ... (370 ms)