Need Help Processing with Image J

Posted by Emily Siska on
URL: http://imagej.273.s1.nabble.com/Need-Help-Processing-with-Image-J-tp5017774.html

Hello,

I have SEM images of rubber fragments that I would like to process with Image J and I have been having a few problems. The rubber pieces do not have much contrast with the carbon tab that they are on and they are also not smooth - so there are lighter and darker spots of a paretic.  When using Image J I adjust the threshold until the particles and background have a high contrast. I try to count and find the perimeter and area of the parcels but the program seems to pick up random shapes on some particles instead of the whole thing. A friend said that its because although I see the particle as just white, the program is picking up differences and treating them like small individual particles. Is there any way around this?


Thank you for all your help.

~Emily Siska
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